Monday, July 23, 2018

Synthetic Botox might help to treat Chronic Pain

Synthetic Botox molecules might help to treat Chronic Pain

Fantastic news for the Botox’ friends, this amazing molecule is not only enhancing your appearance and making you look younger and prettier, it can also reduce pain!
This month, a group of researchers from the UCL, the University of Sheffield and the Hospital for Sick Children, published a groundbreaking study showing how a derivate of Botox can provide long-lasting pain relief in mice without adverse effects.
The researchers mixed an opioid (dermorphin) with a variant of the botox molecule to create “Derm-BOT” and the molecule SP-BOT (a substance P-modified botulinum molecule). Then, they injected these molecules into the spine of different mice and found that they target and silences pain signals from neurons in the spinal cords of mice. One of the authors of the study, Dr. Steve Hunt (from UCL Cell & Developmental Biology), explained that Derm-BOT doesn't affect muscles like the botulinum toxin used to reduce wrinkles but it does block nerve pain for up to four months without affecting normal pain responses. More positive features of Derm-BOT are that it is safe to manufacture, is non-toxic and doesn’t kill neurons. The study covered 5 years and 200 mice that were treated with a single injection of Derm-BOT, SP-BOT or morphine. The behavior of the mice was monitored to track their pain-response. Both SP-BOT and Derm-BOT have a long-lasting effect in both inflammatory and neuropathic pain model, successfully silencing neurons without cell death.
Chronic pain affects more than 25 million Americans and is related to reduced life span, anxiety, and depression. Given the current opioid crisis, aka abuse, addiction and overuse of prescribed opioid painkillers, having optional treatments for chronic pain is essential. Moreover there is little evidence that opioids have along lasting effects maybe because the body builds up a tolerance to repeated drug use over the long term. Some reports indicate that opioids may increase the body's sensitivity to pain. On the other hand, a single injection of Derm-BOT reduced mechanical hypersensitivity to the same extent as morphine. More studies in mice and a follow up on humans will help to understand and progress this new treatment that brings hope to the patients suffering chronic pain.


Reference
Selective neuronal silencing using synthetic botulinum molecules alleviates chronic pain in mice
Maria MaiarĂ¹, Charlotte Leese, Michelangelo Certo, Irene Echeverria-Altuna, Antonina S. Mangione, Jason Arsenault, Bazbek Davletov and Stephen P. Hunt

Science Translational Medicine 18 Jul 2018:
Vol. 10, Issue 450, eaar7384
DOI: 10.1126/scitranslmed.aar7384

Saturday, July 7, 2018

Can Chocolate be Good for You? The Dark and Light Side of the Force

Can Chocolate be Good for You? The Dark and Light Side of the Force
By Jesica Levingston Mac leod, PhD

It is this time of the year again: World chocolate day – the best excuse to give and more importantly to EAT a lot of chocolate. But, maybe a better gift that receiving chocolate, is to know that eating chocolate might be good for your health.

In the beginning chocolate was “created” as a medicine – a healthy beverage – around 1900 BC by Mesoamerican people. The Aztecs and Mayas gave it the name of “xocolatl”, it means bitter water, as the early preparations of the cacao seeds had an intense bitter taste. Almost one year ago, a longitudinal study, done in the US East Coast, connected eating chocolate with better cognitive function. Yay! Great news, right? The scientists gathered information over a period of 30 years (starting in 1976) from 968 subjects (aged 23-98 years) in the Syracuse-Maine area. The results showed that more frequent chocolate consumption was meaningfully associated with better performance on the global composite score, visual-spatial memory and organization, working memory, scanning and tracking, abstract reasoning, and the mini-mental state examination. Importantly, they pointed out that with the exception of working memory, these relations were not attenuated with statistical control for cardiovascular, lifestyle and dietary factors across the participants.

More good news arrived last summer: an Italian research team announced that flavanol-rich chocolate improves arterial function and working memory performance counteracting the effects of sleep deprivation. The researchers investigated the effect of flavanol-rich chocolate consumption on cognitive skills and cardiovascular parameters after sleep deprivation in 32 healthy participants, who underwent two baseline sessions after one night of undisturbed sleep and two experimental sessions after one night of total sleep deprivation. Two hours before each testing session, participants were assigned to consume high or poor flavanol chocolate bars. During the tests the participants were evaluated by the psychomotor vigilance task and a working memory task, systolic blood pressure (SBP) and diastolic blood pressure (DBP), flow-mediated dilation and pulse-wave velocity. As you might know, sleep deprivation increased SBP/DBP. The result was that SBP/DBP and pulse pressure were lower after flavanol-rich treatment respect to flavanol-poor treatment sleep deprivation impaired flow-mediated dilation, flavanol-rich, but not flavanol-poor chocolate counteracted this alteration. Flavanol-rich chocolate mitigated the pulse-wave velocity increase. Also, flavanol-rich chocolate preserved working memory accuracy in women after sleep deprivation. Flow-mediated dilation correlated with working memory performance accuracy in the sleep condition.

The European Food Safety Authority accepted the following statement for cocoa products containing 200 mg of flavanols: “cocoa flavanols help maintain the elasticity of blood vessels, which contributes to normal blood flow”. This statement means that flavanol-rich chocolate counteracted vascular impairment after sleep deprivation and restored working memory performance. In another study led by Columbia University Medical Center scientists, dietary cocoa flavanols—naturally occurring bioactives found in cocoa—reversed age-related memory decline in healthy older adults. One possibility is that the improvement in cognitive performance could be due to the effects of cocoa flavonoids on blood pressure and peripheral and central blood flow. Following on this other chocolate attribute, it was shown than weekly chocolate intake may be beneficial to arterial stiffness.

But, there are some bad news! A review of 13 scientific articles on this topic, provided evidence that dark chocolate did not reduce blood pressure. However, the reviewers claimed that there was an association with increased flow-mediated vasodilatation (FMD) and moderate for an improvement in blood glucose and lipid metabolism. Specifically, their analysis showed that chocolates containing around 100 mg epicatechin can reliably increase FMD, and that cocoa flavanol doses of around 900 mg or above may decrease blood pressure if consumed over longer periods: “Out of 32 cocoa product samples analyzed, the two food supplements delivered 900 mg of total flavanols and 100 mg epicatechin in doses of 7 g and 20 g and 3 and 8 g, respectively. To achieve these doses with chocolate, you will need to consume 100 to 500 g (for 900 mg flavanols) and 50 to 200 g (for 100 mg epicatechin). Chocolate products marketed for their purported health benefits should therefore declare the amounts of total flavanols and epicatechin”. The method of manufacturing dark chocolate retains epicatechin, whereas milk chocolate does not contain substantial amounts of epicatechin.

The first epidemiological “indication” for beneficial health effects of chocolate were found in Kuna natives in Panama with low prevalence of atherosclerosis, type 2 diabetes, and hypertension. This fact correlated with their daily intake of a homemade cocoa. These traits disappear after migration to urban and changes in diet.



There are many claims about the potential health benefits of chocolate, including anti-oxidative effect by polyphenols, anti-depressant effect by high serotonin levels, inhibition of platelet aggregation and prevention of obesity-dependent insulin resistance. Chocolate contains quercetin, a powerful antioxidant that protects cells against damage from free-radicals. Chocolate also contains theobromine and caffeine, which are central nervous system stimulants, diuretics and smooth muscle relaxants, and valeric acid, which is a stress reducer. However, chocolate also contains sugar and other additives in some chocolate products that might not be so good for your health.



Oh well, maybe the love of chocolate is like any other romantic affair: blind and passionate. Apparently, the beneficial dosage is 10 g of dark chocolate per day (>70% cocoa), so enjoy it as long as the serotonin boost for rewarding yourself with a new treat last.



Happy world chocolate day!



Monday, April 30, 2018

Ebola – Closer than You Think

Ebola – Closer than You Think


Ebola, the hemorrhagic fever is closer than you think, but there is no reason to panic…yet!

By Jesica Levingston Mac leod, PhD

In case you did not hear about it, the Center of Disease Control (CDC) reported an outbreak of a “more virulent” Ebola virus infections in Guinea, spreading now to Sierra Leone . Ebola virus is the etiological agent of severe hemorrhagic fever. The symptoms? Fever, rash, severe abdominal pain, vomiting, and bleeding, both internally and externally. The fatality rate? Around 90%. Even worse, these outbreaks are occurring with increasing frequency. Some explanations for this are the increased contact between humans and the natural reservoir of the viruses (fruit bats), and fluctuations in viral load and prevalence in this reservoir. The transmission of the virus mostly occurs by contact with infected blood, secretions or organs of either bats, nonhuman primates or humans. This is why you should not eat bats or monkeys if you visit any of the affected areas, or hang around any cemeteries. Not surprisingly, Ebola was named as the most frightening disease in the world. It was documented for the first time in 1976 in the Republic of Congo; one of the sources came from the Ebola River.



In 2012 an outbreak in Uganda found us in a similar medical emptiness: the research of two of the vaccines that were “apparently” going great had been canceled by the department of defense, due to funding constraints. Therefore, so far we do not have any vaccine or effective treatment available.



Albeit a DNA based vaccine was described in 2003 to fully protected macaques against the fatal virus, it did not continue to further clinical trials. It was not until 10 years later that a group in the US National Institutes of Health published research about a vaccine consisting of a recombinant vesicular stomatitis virus expressing the ebola glycoprotein which protects macaques from Ebola virus infections, although this method is not licensed for human use.



But, why does the US department of defense care about an African virus? The answer is pretty obvious: it can be used as a bio hazard weapon. On the other hand, no leading pharmaceutical is going to invest in a “very expensive and time consuming” vaccine development to be used in countries that can not afford even a basic level of health care. Some compounds are showing a promising antiviral effect in vitro and/or an inhibition of a variety of viral proteins activities. Sadly, all of them are in an early stage of drug development.



Before freaking out, the best “cure” and prevention method against this scaring virus is knowledge, so check out the updates in the CDC website.

Chasing the "One Drug" to Rule Them All

Chasing the "One Drug" to Rule Them All
(All meaning all the hemorrhagic fever viruses)

By Jesica Levingston Mac leod, PhD

Almost all the hemorrhagic fever viruses are listed by the World Health Organization to be only handled in Biohazard level 4 facilities, as they are potential agents of bioterrorism. These are also RNA viruses, which have a mortality ratio between 30 to 90% and there are no vaccines or effective treatment methods available. In fact, the recommended anti viral treatment; Ribavirin has not shown to be very successful in a randomized-controlled trial, as Ribavirin was not superior to no Ribavirin treatment in mortality rates. One would think that these types of viruses could only arise in far and remote regions like Argentina (in the case of Junin virus) or Congo (like the Ebola virus). However with the increase in the intercontinental travels these viruses could be closer than what you would expect.

But not all is bad news: Lu et al. examined the effect of inhibitors based on a host protein (Tsg101), and discovered an inhibitor for at least 2 negative stranded RNA viruses (Junin virus and Ebola virus). Compound 0013 could reduce Junin virus egress dissemination and disease progression in infected individuals by inhibiting the viral nucleoprotein-Tsg101 interaction. Moreover, since this Tsg101 related recruitment system is utilized by other RNA virus pathogens (e.g. Ebola virus and HIV-1), the compound 0013 has the potential to function as a broad-spectrum, host-oriented antiviral drug.

In a more advance stage of drug development, in a recent Nature article, Warren et al. showed that a compound: BCX4430, inhibits infection of Ebola and Marburg virus in human cells. Furthermore, the post exposure of this compound in rodents also protects against further viral infections. BCX4430 is a novel synthetic adenosine analogue that inhibits viral RNA polymerase function. The hallmark is that BCX4430 protects macaques from Marburg virus infection when administered as late as 48 hours after infection. Finally, BCX4430 exhibits broad-spectrum antiviral activity against other viruses, including bunyaviruses, arenaviruses, paramyxoviruses, coronaviruses and flaviviruses.



Lastly, in the Science magazine, Andrews et al. showed the efficiency of the compound GSK744 in protecting macaques against HIV infection. This drug is an integrase strand-transfer inhibitor that has been formulated as a long-acting injectable. GSK744 was administered at two time points 4 weeks apart, beginning 1 week before virus administration, and the macaques were challenged weekly for 8 weeks. GSK744, protected all animals against repeated low-dose challenges. These results suggest that GSK744 could potentially decrease adherence problems associated with daily preexposure prophylaxis and may be administered quarterly in humans. And, of course, this compound has been suggested to be use as an inhibitor for other RNA viruses.



As you can see, not all the researchers involved in drug discovery are only focus in their own virus of interest or are as avid to put the drugs in the market before passing with honors all the FDA approval test, as the movie “Dallas Buyers Club” showed us recently.



The drug discovery process is very extensive, exhausting and expensive; in order pass the reach the pre-clinical and clinical testing to pass the stringent FDA approval. Therefore, finding “the drug” than can be effective against a variety of viruses (and not toxic for humans!) could be a shortcut in medicine development.

8 Ways to Get the Best out of Networking Events

8 Ways to Get the Best out of Networking Events


By Jesica Levingston Mac leod, PhD

Para la traduccion en espanol mirar mas abajo.

When you are attending a networking event, it doesn’t matter if you are hiring, searching for a job or just increasing your LinkedIn connections, there are some simple ideas that would make you go home with a smile on your face and a lot of business cards in your pocket.



1. Know what are you looking for, as always in life
You must have your aim or goal in mind, so you can transmit it to the other people. Prepare a short introduction about yourself and your expectations in advance, and when I say short, I mean it. Nothing is more boring than a stranger giving you a dissertation on a topic that you do not care about. Therefore focus your short introductory speech according to your goal for the event. On the other hand, the “hit and run” strategy is a highway to failure. For example if you are job hunting, it is important to sell yourself to the correct people… which brings me to the next topic..



2. Connect smart!
If you start a conversation and it does not look very productive for you, do not be afraid and just say “thanks, bye” and move on, no harm done. Normally the networking events don’t last more than 2 hours and you have to take advantage of every available second.



3. Know the attendants
So try to get a list of the attendants before hand, and “Google” them in order to know if they’re important connections for you and which kind of conversations or common interest you can discuss. This is not stalking, but sometimes it is good to check out photos of people you want to talk to so you can recognize them. Moreover, leave your mark, tell then something that will make then remember you, and if you can: find a reason to keep in touch.



4. Have an ice breaker
Some tips for those shy souls: just go and say, “Hi, how are you?” and honestly wait to get an answer. You can follow it with “I am John Doe, I work in Awesome-land, what are you doing?”. Or you can ask questions such as, “do you work in….?” or “What are you drinking?” “What advice would you give to someone who wants to break into this field?” “Would you recommend with who I should speak? May I use your name as a reference?” I know it is kind of a cheesy icebreaker but it is the best shot. Everybody is there to meet people, and if you just stand around staring at the empty space the probabilities of you meeting another interesting human being are very low.



5. Dress to impress, but not too much
About the look: dress your best according with the type of event you are attending, but always let your own style show through. It’s your personality that makes you special and different, and that can be reflected in your outfit.



6. Be special
Write your name on the tag in big, clear letters (young people forget that old people can’t read small writing) and put it on the right side of your chest. Why? Because when you shake someone’s hand your right shoulder will be pointing directly at them. Also most of the people you will meet are right handed and the easier for them is to put the sticker on their left side, so you might have a possible conversation starter.

[box style=”rounded”]What about a “wing man”? Personally, I love this strategy because I team up really well with my friends, but it can be contra productive as the people can feel it overwhelming to have a crowd “attacking” them.[/box]


7. Be brave
The most important advice is to just go. Event if you are afraid of putting yourself thorough this wild networking event world, just do it. I was searching for a job when a friend convinced me to attend a biotech event at a bar. The event was not looking very successful when we had first arrived and I was regretting my decision. But, eventually a handsome man walked over to me and mentioned in a very friendly way that he was working for a company that was looking for a chemist, which I am not. I literally moonwalked far away from him as I didn’t want to waste my time. But before leaving he came back again and handed me his card, which I added to the pile. Two weeks later I updated my LinkedIn connections adding all the professionals that I met in these events and to my surprise a message came back from this handsome man inviting me for a coffee. Long story short: I met my boyfriend in a networking event. So you never know what can came out of these gatherings. One thing is sure, only positive things are born from networking. Sharing you experience, knowledge, needs and future goals with other professionals is always rewarding.



8. Let’s listen to professional advice by recruiter Nick Corcodilos
[quote style=”boxed”]True networking is when you spend time with people who do the work you want to do, talking shop. Good networking involves working with other active professionals, even if it’s on a volunteer project, or to learn something new. Good networking is rubbing elbows and enjoying talk and activities related to the work you want to do. Here’s the thing that confuses people and frustrates them: They think we network to get our next job. That’s absolutely wrong. We network to get smarter, to make new friends, to build our value and our credibility in our professional community, to help others, and to enjoy our work outside of the job. Job opportunities arise out of networking; they are not the reason to do it.[/quote]

References:

Nick Corcodilos, in Ask The Headhunter newsletter, “Too late to network?” March 18, 2008.

Where to start:

[unordered_list style=”tick”]

LinkedIN
Networking meetings in NYC – or search Eventbrite for events in your city
NetParty – networking with a twist
The NYC Business Networking group (search or start meetup for similar events near you)

Clone wars – GMOs: Jedis or Siths?

Clone wars – GMOs: Jedis or Siths?


By Jesica Levingston Mac leod, PhD

In any molecular biology lab cloning is a daily procedure, now getting those clones outside of the lab is the huge issue. Genetic modified organisms or GMOs were subjected to specific genes alterations, and then cloned to obtain a larger number of identical organisms. Here, I would like to compare the two faces of this technology and its impact in the nature.



GMOs as Jedis, the good use of the force:

Since this technology was introduced to the field, the pesticide spraying has been reduced by 499 million kg (-8.7%) and this decreased the environmental impact associated with herbicide and insecticide use on the crops by 18.6% (as measured by the indicator the Environmental Impact Quotient [EIQ]). Furthermore, it has been reported a significant reduction in the release of greenhouse gas emissions from this cropping area, which, in 2012, was equivalent to removing 11.88 million cars from the streets.

Economically, they bring a high advantage to the farmers, allowing them to grow in a competitive environment, generating more products with a lower expenses.

GMOs are helping to supply resources to a never ending growing world population. Therefore, they could be a solution for the doomsday prediction that the economist Maltus made more than 100 years ago: “we are going to run out of resources and we won’t feed an exponential rising world population”. Science published at the beginning of 2000 a breakthrough research: the golden rice. This special GMO counts with the addition of three beta-carotene biosynthesis genes. These compounds added nutrient value to the rice, as they are precursors for the vitamin A biosyntheses. This project was leaded by Drs. Ingo Potrykus and Peter Beyer of the University of Freiburg, whom had the aim to introduce this enriched rice in the african, latin american and asian market where the deficit in this vitamin causes terrible health problems. At the time of publication, golden rice was considered a significant breakthrough in biotechnology, as the researchers had engineered an entire biosynthetic pathway. Five years later, a new version of the golden rice producing up to 23 times more beta-carotene than the original, was announced.

GMOs on the dark side of the force:

As an artificial organism that we are introducing to nature we can only try to predict how are we going to impact the environment. The ecology of this artificial selection was predicted as catastrophic, for example for the soy harvest in Argentina, where the excessive use of this GMO leaves the soil without nutrients, kind of “death” and unable to generate any other product. This exhaustion of the field may bring a negative impact in the future.

According to the center for food safety, GMOs products make up about 90 percent of cash crops like cotton, corn and soybeans nationwide. As Monsanto holds the 80% and the 90% of american corn and soybeans , respectively, and its licenses, the monopoly issue started to rise. Neither the farmers or scientist are allowed to research on the GMOs created by Monsanto, without a legal permission. This avoids the independent safety testing, and some scientists have rise the case to the US Supreme Court.

Furthermore, the farmers are subject to pay the increase price for the seeds that they can only buy for a few companies. Indeed, between 1995 and 2011, the per acre cost of corn and soybean increased 259% and 325%, respectively (US Department of Agriculture). With this strong license policy, an increasing number of small farmers have gone bankrupt as a consequence of having an accidental (like wind dispersal, split seed or cross contamination) presence of GMO on their fields. It is not surprising that with this situation the idea of changing seeds, buying a non-GMO species scares the farmers.



The labeling topic is even more sensible. The Food and Drug Administration favors voluntary labeling and says GMO products must meet the same safety levels as other foods. On the other hand the Center for Food Safety supports mandatory labeling. The GMOs producers prefer to avoid the labeling, as it brings unwanted attention to the product and bad advertising. The pro labeling organizations claim that it is the consumer right to know exactly what they are eating. My favorite comment in this regard was made by Gene Hall, a spokesman for the Texas Farm Bureau: “We don’t need to label something that is absolutely safe.”

As a great technology in development, GMOs are like Anakin Skywalker in his early age trying to decide which side of the force he should join, both present advantages and disadvantages, but without a correct guidance, like Yoda would be, this technology could be joining the dark side of the force.


Fasting Can Make You Healthier

Fasting Can Make You Healthier


By Jesica Levingston Mac leod, PhD

Believe it or not, breakthrough new research has shown that fasting could be good for you. The article was indeed featured in the Nature journal and the impact of this study relies on the conclusion that fasting promotes haematopoietic stem cell (HSC) function. Stem cells are good for you because they can differentiate into specialized cells and can divide to produce more stem cells.

I personally challenged myself by fasting during Ramadan. Ramadan is one of the pillars of the Muslim religion. It consists of fasting during a month from sunrise to sunset in order to reflect the essence of piety and to be aware of the plight of the underprivileged. Other cultures include fasting in their practices. In the Jewish religion the fasting day is named Yom Kippur, the Day of Atonement. It is described as a Jewish festival without food, but full of praying, introspection and self-judgment.

During my fasting period, my friends noticed an off character onset of passive aggressiveness in me, and indeed I was pretty cranky… and super hungry. One of my favorite comedians, Luis CK, once said that we incorrectly overuse the “I am starving” phrase, while people in Africa are really dying for starvation… so I won’t say I was starving, but certainly I was in a glucose deprived state of mind, which was affecting my behavior.

The most challenging part for me was being dehydrated, as you should also fast liquids during Ramadan. Contrary to the great health guru; the actress Cameron Diaz, who taught in her book that drinking plenty of water is the basis for a healthy body, fasting liquids seemed counterproductive in my experience.

Fasting is often indicated in general medical practice particularly prior to surgery or other procedures that require general anesthetics, because of the risk of pulmonary aspiration of gastric contents after induction of anesthesia (i.e., vomiting and inhaling the vomit, causing life-threatening aspiration pneumonia). One should also fast if undergoing a cholesterol or glucose test, as these measurements require a 12 hour fasting period so that a baseline can be established. These acute/short fasting periods are generally safe.

What more, a study in mice published in 2008 showed that short-term fasting (less than 48 hours) is effective in protecting normal cells but not cancer cells against high dose chemotherapy. The following year another study published in Science proved that caloric restriction delays disease onset and mortality in rhesus monkeys. In a human study, including 10 cancer patients under chemotherapy, Sadfie and collaborators did not report significant side effects caused by fasting alone other than hunger and lightheadedness. In this study all patients voluntarily fasted for a total of 48 to 140 hours prior to and/or 5 to 56 hours following chemotherapy administered by their treating oncologists. In those patients whose cancer progression could be assessed, fasting did not prevent the chemotherapy-induced reduction of tumor volume or tumor markers. Fasting was well-tolerated and was associated with a self-reported reduction in multiple chemotherapy-induced side effects, suggesting that fasting in combination with chemotherapy is feasible, safe, and has the potential to ameliorate side effects caused by chemotherapies.



In the significant article that I mentioned before, Chen and collaborators showed that prolonged fasting (PF), exceeding 48 hours, activates a metabolic switch to lipid- and ketone-based catabolism and decreases circulating insulin-like growth factor-1 (IGF-1), which has been shown to reduce chemotoxicity (1) How? They couldn’t find an answer yet. However they clearly demonstrated that the decrease of circulating IGF-1 in the blood was accompanied by a reduction in protein kinase A (PKA) pathway activity in a variety of cell types. PKA has several functions in the cell, I.e. regulation of glycogen, sugar, and lipid metabolism and it regulates other proteins with a valuable role in stem cell stress resistance, self-renewal and pluripotency maintenance.

Interestingly, when Chen and collaborators exposed mice to cycles of prolonged fasting followed by challenges with cyclophosphamide (a drug used in chemotherapy), they noticed the reduction in the mortality and apoptosis (programmed cell death) of long- and short-term HSCs as well as multipotent progenitors in the bone marrow. In addition, multi-lineage differentiation was improved in these animals compared with fed mice, in vitro and in transplantation experiments. These positive effects of prolonged fasting were independent of the chemotherapy treatment, as they were also present in aged animals, which naturally exhibit a reduction in HSC function and multi-lineage potential. The effects of prolonged fasting could be reproduced in mice lacking the growth hormone receptor, which also have low levels of IGF-1. Transplantation experiments showed that low levels of IFG-1 in animals led to a reduction in IGF-1-mediated PKA signaling, both in haematopoietic cells and in associated stromal cells. Strikingly, the researchers could restore haematopoietic function by reducing the levels of either IGF-R1 or the PKA catalytic subunit. Conversely, the benefits were abolished if exogenous IGF-1 was added.



The scientific community is excited about these findings, and we hope understanding the positive effects of fasting can have implications in improving the quality of life of cancer patients… and all the humanity in general. On the other hand, I must cite one of the best Americans: “He that lives upon hope will die fasting”, Benjamin Franklin.